High-Performance Ion Chromatography (HPIC) is a type of ion chromatography that provides rapid and high-resolution separation of ions. In ion chromatography, charged particles (ions) are separated based on their interaction with a charged stationary phase. This type of chromatography is used primarily for the separation and quantification of anions and cations in a wide variety of sample matrices.
The principle of HPIC is the same as other chromatographic techniques where a sample is introduced into a stream of mobile phase and passed through a column packed with stationary phase. The ions in the sample interact with the stationary phase based on their charge, size, and shape, resulting in a separation based on the time it takes for each ion to pass through the column (retention time).
HPIC offers several advantages including high resolution, sensitivity, and speed of analysis. It can detect and quantify trace levels of ions in complex matrices, making it suitable for a wide range of applications.
Role in Analysis Services:
Environmental Analysis: HPIC is extensively used for the analysis of environmental samples. It can quantify anions and cations in water (surface water, ground water, wastewater, etc.), soil, and air samples. Common analytes include fluoride, chloride, nitrite, nitrate, sulfate, and phosphate, as well as alkali and alkaline earth metals.
Food and Beverage Analysis: HPIC can analyze food and beverage samples for various ions. It can be used to quantify nutrients, preservatives, and other additives, or to monitor the consistency of these components in quality control.
Pharmaceutical Analysis: HPIC can be used to analyze pharmaceutical products for their active ingredients, excipients, impurities, and degradation products. It can also be used to study the drug’s stability and monitor manufacturing processes.
Industrial Analysis: In industrial processes, HPIC can be used to monitor chemical reactions, analyze process streams, and control quality.
Clinical Analysis: HPIC can be used to analyze biological samples such as blood and urine for a variety of ions related to physiological and pathological conditions.
In conclusion, HPIC plays a crucial role in analytical services, given its ability to provide rapid, sensitive, and high-resolution analyses of a wide variety of samples. By separating and quantifying ions, HPIC aids in environmental monitoring, quality control for food and beverages, pharmaceutical development and manufacturing, industrial process control, and clinical diagnostics.